Gene Expression Profiling of WT and KDM3A Knocked out Cell

We identified KDM3A, a demethylase of histone H3K9me1/2, as a positive regulator for hippo target genes. We found that H3K27ac upregulation is highly correlated with gene activation, but not H3K4me3; and transcription repression of certain TEAD1 target genes, such as BBC3, is important for the pathway function. KDM3A knockout caused upregulation of H3K9me2 mainly on TEAD1-binding enhancers rather than gene bodies, leading to decrease of H3K27ac and TEAD1 binding on enhancers and impaired transcription. KDM3A was knocked out in HCT116 and the gene expression profile was studied by RNA sequencing Please note that, in the sample titles, M represents Mock, which means cell cultureed in normal state. S represents Starvation, which mens cells growed up in normal culture in a due time, then change the nomal culture into no-serum culture. S+F means addition of FBS into the no-serum culture after cell-starved for for 24 hours.