Immune profiling of lymph nodes drained to cold vs hot triple-negative breast tumors.

NanoString pan-cancer immune panel was used to quantified the expression of 730 immune genes of 15 tumor-free TDLNs and paired tumors. FFPE slides were used to quantified the gene expression. We collected and analyzed 7 cold tumor-TDLNCold pairs and 8 hot tumor-TDLNHot pairs. FFPE samples were subjected to NanoString analyses. By using the NanoString Pan-Cancer immune panel, we quantified the expression of 730 immune genes of these 15 tumor-free TDLNs and paired tumors. Stromal TIL percentage of each tumor was scored by a pathologist from H&E-stained slides. Tumors were graded as cold (<10% stromal TIL), hot (>60% stromal TIL), or intermediate (10-60% stromal TIL).