Determination of sensitivity and specificity of RT-PCR assay for N. meningitidis (ctrA), S. pneumoniae (lytA), and H. influenzae (bexA) in cerebrospinal fluid and serum specimens (see text for details).

*Number of specimens contributing to the calculation of sensitivity or specificity**Specificity determined using specimens positive for other organisms***Specificity determined using specimens positive for other organisms or culture negative****Specificity determined using specimens positive for other organisms or culture negative but positive for second gene target