Data from: Evolution of ecological dominance of yeast species in high-sugar environments
收藏Mendeley Data2024-06-25 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.k3f17
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资源简介:
Pyrosequencing competition dataCalibrated pyrosequencing data from Competition 1. The frequency of each competitor species in either paired competition with S. cerevisiae or in pooled monocultures. The adjusted frequency is based on calibration curves and eliminates negative values.CalibratedPyrosequencingData.csvSequencing competition dataCalibrated sequencing data from Competition 2. The frequency of each competitor species in competition with different reference strains, along with concatenated barcode and indexes used for sample ID. The fitness difference and experimental metadata is also included.biodiversity.data.csvHigh-sugar environmentData from assays (gren01 and nut01) to determine the intrinsic growth rate of each yeast species in high-sugar environments including grape juice. The OD600 (cell density) is given for time-points between zero and 48 hrs.grape_environment.csvEthanol resistanceData from assay to determine the ethanol tolerance of each yeast species. OD600 (cell density) is given for time-points between zero and 48 hrs for different concentrations of ethanol. One sample was omitted from the analysis (Smik, Rep C, 2% ethanol) due to possible contamination from a pipetting error that occurred during the experiment.ethanol.csvSupernatant inhibitorsData from assay to determine whether Saccharomyces cerevisiae produces unknown compounds that inhibit the growth of other yeast species. OD600 (cell density) is given for for time-points between zero and 48 hrs in supernatant cultures. A small number of samples (24/432) registered growth in un-innoculated controls before 36 hours, and these samples were omitted from our analysis. Following the removal of those samples, Lachancea kluyveri lacked sufficient control samples for analysis and was not included in the analysis.supernatant.csvSequencing metadataBarcodes, indexes and metadata used to extract species tags from raw sequencing data for BITS-3 and BITS-4 libraries.Metadata.xlsBITS-3 library readRaw sequence data with reads containing barcode, linker then species specific sequences.BITS-3_S1_L001_R1_001.fastq.gzBITS-3 library indexRaw sequence data with reads containing indexes.BITS-3_S1_L001_I1_001.fastq.gzBITS-4 library readRaw sequence data with reads containing barcode, linker then species specific sequences.BITS-4_S1_L001_R1_001.fastq.gzBITS-4 library indexRaw sequence data with reads containing indexes.BITS-4_S1_L001_I1_001.fastq.gz
创建时间:
2023-06-28



