Meta-analyses of discovery and replication cohorts.

Effects size is given per minor allele. All seven discovery cohorts (n = 8,938) were included in the GWAS of chromosomes 1–22 while only the two largest cohorts (FHS and SHIP. n = 5,067) had GWAS data available for the X chromosome. A1 = allele 1. A2 = allele 2. FREQ* = Frequency of allele 1. In the KORA cohort, testosterone was measured using plasma but the analyses after excluding KORA yielded similar results. Calculated free testosterone was calculated for all subjects with both testosterone and SHBG available by using a modified law of mass action equation. The concentrations of testosterone and SHBG and a fixed value for SHBG's dissociation constant were used in these calculations.