Gul Civelekoglu-Scholey, Li Tao, Ingrid Brust-Mascher, Roy Wollman, Jonathan M. Scholey (2011) CIL:30567, Drosophila melanogaster, early embryonic cell. CIL. Dataset

A confocal 4D-stack from a transgenic Drosophila embryo during prometaphase expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and the lamin-B tail dominant negative subfragment. The microinjection of a mixture of the lamin-B tail dominant negative subfragment disrupts the nuclear lamina and causes defects in mitosis. This image is original data contributing to Fig. 6 "Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

一帧来自转基因果蝇胚胎的共聚焦四维图像，该胚胎在前期表达层蛋白-GFP（绿色），并注射了罗丹明标记的微管（红色）及层蛋白-B尾部显性负性亚片段。层蛋白-B尾部显性负性亚片段的显微注射破坏了核纤层，导致有丝分裂缺陷。本图像为原图，是Civelekoglu-Scholey等人（2010年）发表的论文《通过解聚层蛋白-B包膜增强的拮抗性动力依赖性力平衡维持前期纺锤体稳定性》中图6“层蛋白-B包膜的机能障碍干扰纺锤体长度变化和有丝分裂完成”的贡献。该论文发表于《细胞生物学杂志》（J. Cell Biol.），第188卷第1期，页码49-68。