Comparative Transcriptomic Analysis of Dermal Wound Healing Reveals De Novo Skeletal Muscle Regeneration in Acomys cahirinus

This project is a comprehensive analysis of gene expression following full-thickness excisional dermal wounds in the African spiny mouse and the laboratory mouse. 8mm excisional wounds were made on the dorsal skin. Wounds were harvested on day 7 and 14 after wounding. Normal, unwounded (day 0) skin was used as a control. N=4 for each time point for each species. 2x150 bp paired-end reads. DSN library was equal amounts of all Acomys samples, pooled, and normalized using duplex-specific nuclease. The 'Mus_Day-0_rep3' sample was removed from downstream analysis due to poor QC results, and is not included here.本项目针对非洲刺毛鼠（African spiny mouse）与实验室小鼠的全层切除性皮肤创伤后的基因表达展开全面分析。研究人员在两组实验动物的背部皮肤制备直径8mm的切除性创面，分别于创伤后第7天和第14天收集创面组织；以未受创伤的正常皮肤（创伤后第0天）作为对照样本。每个物种的每个时间点设置4个生物学重复（N=4）。测序采用2×150 bp双端测序读长（paired-end reads）。针对所有非洲刺毛鼠（Acomys）样本，将其等量混合后构建DSN文库（DSN library），并通过双链特异性核酸酶（duplex-specific nuclease）完成文库均一化处理。由于质量控制（QC）结果不合格，"Mus_Day-0_rep3"样本被排除在后续分析之外，本数据集未收录该样本。