Fitness profiling using the yeast deletion library to determine contribution of all non-essential genes to survival under acute exposure to lethal ethanol stress

To determine the contribution of yeast genes to acute lethal ethanol stress, we performed fitness profiling of a pooled haploid yeast deletion library. This deletion library contains 4,500 deletions of most non-essential yeast genes. Comparing the abundance of post-stress gene deletions to pre-stress gene deletions allows for the quantification of the effects of each gene deletion on survival为探究酵母基因在急性致死乙醇胁迫中的贡献，我们对混合单倍体酵母缺失文库开展了适应度谱分析（fitness profiling）。该缺失文库涵盖了绝大多数非必需酵母基因的4500个缺失突变体。通过对比胁迫后与胁迫前的基因缺失株丰度，可量化各基因缺失对菌体存活的影响。