Bacteria contaminants detected by organic inverter-based biosensors

This study presents a novel rapid bacterial detection method employing an organic complementary metal–oxide–semiconductor (O-CMOS) inverter fabricated with pentacene and PTCDI-C13. Various bacteria, including Escherichia coli (E. coli) O157, Staphylococcus aureus (S. aureus) ATCC25922, and Enterococcus faecalis (E. faecalis) SH-1051210, were tested on the inverters operating at an ultra-low voltage of 2 V. Analysis of the electrical signals revealed a discernible shift in the inverter's switching voltage attributed to the negative charge density on the surfaces of bacteria. This voltage shift exhibited a direct correlation with bacterial concentration, thereby enabling the direct measurement of concentration without the need for external devices. The inverter-based sensor demonstrated a linear response across bacterial concentrations ranging from 102 to 108 CFU/mL, with a sensitivity exceeding 60%. Its primary advantage over existing detectors lies in its ability to directly read the switching voltage, facilitating rapid and accurate measurements, and offering significant potential for mass production.

本研究提出了一种新颖的快速细菌检测方法，该方法采用由戊二烯和PTCDI-C13构成的有机互补金属氧化物半导体（O-CMOS）逆变器。对包括大肠杆菌（E. coli）O157、金黄色葡萄球菌（S. aureus）ATCC25922和粪肠球菌（E. faecalis）SH-1051210在内的多种细菌进行了测试，测试在2伏的超低电压下进行。对电信号的解析揭示了逆变器切换电压的明显偏移，这种偏移归因于细菌表面的负电荷密度。这种电压偏移与细菌浓度呈直接相关性，从而能够直接测量浓度，无需外部设备。基于逆变器的传感器在102至108 CFU/mL的细菌浓度范围内表现出线性响应，灵敏度超过60%。相较于现有检测器，其主要优势在于能够直接读取切换电压，从而实现快速精确的测量，并具有大规模生产的显著潜力。