Expression Profiles of E11.5 Mouse Optic Fissure

Mice that are mutant for both Fgfr1 and Fgfr2 specifically in the developing retina develop coloboma. To analyze the transcripts that are affected by defective FGF signaling, we micro-dissected the optic fissure region from the control and FGFR condtional mutant mice and did microarray analysis. E11.5 control and Six3Cre+; Fgfr1fx/fx;Fgfr2 fx/fx optic fissures are dissected using laser-assisted micro-dissection microscope, RNAs are extracted and labelled and hybridyzed to chips在发育中的视网膜中特异性携带成纤维细胞生长因子受体1（Fgfr1）与成纤维细胞生长因子受体2（Fgfr2）双突变的小鼠会出现脉络膜视网膜缺损（coloboma）。为了探究受功能异常的成纤维细胞生长因子（Fibroblast Growth Factor, FGF）信号通路影响的转录本，我们从对照组及FGFR条件性突变小鼠中显微解剖了视裂区域，并开展了微阵列分析（microarray analysis）。本研究利用激光辅助显微切割显微镜（laser-assisted micro-dissection microscope），对胚胎发育第11.5天（E11.5）的对照组小鼠与Six3Cre+; Fgfr1fx/fx; Fgfr2fx/fx基因型小鼠的视裂组织进行解剖，随后提取核糖核酸（RNA）并完成标记，将其与基因芯片进行杂交。