Escape from X inactivation is directly modulated by Xist non-coding RNA [Astrocytes]

X-chromosome inactivation (XCI) silences one copy of the X-chromosome in female cells, but a number of genes escapes XCI. We asked whether increased levels of Xist RNA, the molecular actor driving X-inactivation, impacts expression levels of escapees in Astrocytes. To this end, we performed RNA-seq analysis profiling of X-linked genes upon doxycycline-induced Xist upregulation and following doxycycline washout. Overall design: Astrocytes were generated by differentiating NPC clone E6 (C57BL6/J x CAST/EiJ). Doxycyclin treatment for 3 and 7 days (and following wash-out) was performed and allele-specific RNA expression was measured.