A) Chromatins were prepared from Cdx2-inducible ES cells cultured for 48 - 60 hours in the Dox+ and Dox- conditions. Chromatin immunoprecipitation (ChIP) was carried out by using anti-FLAG M2 affinity
To investigate the genetic loci bound by MafB in the intestinal epithelium, we used CUT&RUN. Overall design: To investigate the genetic loci bound by MafB in the intestinal epithelium, we used CUT&RUN
NKX2-1 transcription factor binding site (TFBS) analysis. First, we used HOMER to identify predicted NKX2-1 binding sites (using the HOMER built in matrix “nkx2.1.motif”) across the GRCh38 genome, and
