RNA polymerase binding sites revealed by ChIP-chip in S. Typhimurium wild-type, ΔrelAΔspoT (ppGpp0), and ΔdksA strains at early stationary phase (ESP) and late stationary phase (LSP). Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

S. Typhimurium parent, ppGpp0 and dksA strains were grown to OD's of 2.3 (ESP) and 4.2 (LSP) and subjected to ChIP-chip analysis at 60 nt resolution. Overall design: 38 samples (parent strain, ΔrelAΔspoT, ΔdksA grown to ESP or LSP, protein-DNA crosslinked and fragments immunoprecipitated with Ab against E.coli RNAP beta subunit (Neoclone, cat # WP023), or minus Ab as control. DNA fragments purified & labelled with dCTP-Cy5 and hybridised against Cy3-dCTP labelled gDNA on OGT 4 x 44K 60 mer S. Typhimurium SL1344 array).