Time-Resolved Assembly of pH-Responsive Cubosomal Nanocarriers for Gastrointestinal Delivery of Aloe Vera-Derived Acemannan

The proposal aims to study the pH-responsiveness of composite cubosome carriers encapsulating acemannan using time-resolved synchrotron SAXS. Acemannan is a bioactive from Aloe vera with anticancer, antibacterial, antioxidant, and immunological properties. The cubosomes will have hybrid lipid-biopolymer assemblies with Im3m cubic symmetry. Lipid monoolein will be coated with chitosan-N-arginine and alginate polysaccharide shells, making the nanocarriers pH-responsive for gastrointestinal applications. TR-SAXS will examine structural changes when exposed to gastric and intestinal pH conditions. This will provide insights into the reactivity and stability of these hybrid lipid-polymer nanomaterials as bioactive delivery systems. The study will also explore the effects of pH-sensitive polysaccharides on cubosome structure and stability. Overall, this research will evaluate the potential of these carriers as pH-responsive delivery systems for acemannan and other bioactive compounds.