Breast cancer heterogeneity

Tumor heterogeneity is generated through a combination of genetic and epigenetic mechanisms, the later of which generate stem like cells responsible for tumor formation and metastasis. Although the development of single cell transcriptomic technologies hold promise to deconvolute this complexity, a number of these techniques have limitations of depth and potential bias. We adopted deep and full-length single-cell RNA sequencing to study the cellular and transcriptomic heterogeneity of SUM149, an ER-/PR-/HER2- triple negative breast cancer (TNBC) cell line.