Single cell RNA-seq for human embryonic stem cells and induced pluripotent stem cells
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA631808
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资源简介：
Single cell RNA-seq for human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Single cell RNA-seq using the 10x platform for WIBR3 hESCs and c11/S0730 iPSCS (Zhou et al., 2011; JASN). Cells were grown under typical HESC/iPSC growth conditions: mTeSR1 (Stemcell technologies: 85850). All cells were grown on Matri-gel (Corning: 354277) pre-coated plates for at least half an hour at 37C. Medium was replaced every 24 hours. Cells were passaged by single-cell Accutase (SIGMA: A6964) every 5 days. Single cell RNA-seq was performed using the 10x platform according to the manufacturers instructions.
创建时间：
2020-05-11
