Replication data for: NKCC1 Inhibition Improves Sleep Quality and EEG Information Content in a Down Syndrome Mouse Model

This dataset comprises EEG/EMG data acquired from a population of wild type and Ts65Dn mice being administered either vehicle or bumetanide treatment. The baseline is recorded over 24 hours, then a 6-hours sleep deprivation is performed and the following 18-hours recovery recorded. Data were recorded through the Dataquest A.R.T. telemetry system (Data Science International). Signals were digitized at a sampling rate of 500 Hz with a filter cut-off of 50 Hz. EEG signals were filtered at 0.3 Hz (low-pass filter) and 0.1 kHz (high-pass filter). The polysomnographic recordings were visually scored offline using SleepSign software (Kissei Comtec Co. Ltd, Japan) per four second epoch window to identify wakefulness, non-REM or REM sleep stages. Scoring was performed by an observer blinded to the experimental groups. Specifically, Wake, non-REM and REM states were scored when characteristic EEG/EMG activity occupied 75% of the epochs. EEG epochs determined to have artefact (interference caused by mouse scratching, movement, eating, or drinking) were excluded from the analysis. Artefact comprised <5-8% of all recordings used for analysis. More details can be found in the attached Readme.txt file.