RNA-Seq and RNA Polymerase II ChIP-Seq of mouse spermatogenesis
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
To characterize gene expression in spermatogenesis we sequenced eight mRNA samples from testes of juvenile mice aged between 6 and 38 days post partum. We then classified gene expression profiles and found over 1,000 meiotically-expressed protein-coding genes that have not been previously reported in relation to spermatogenesis. Furthermore, we developed an in silico de-convolution approach to estimate cell type-specific gene expression from temporal gene expression profiles and applied it to our dataset. To further characterize transcription during spermatogenesis, we determined RNA polymerase II (RNAPII) distribution along the genome using ChIP-Seq.
提供机构：
National Institutes of Health
创建时间：
2022-02-20
