Environmental DNA metabarcoding to monitor tropical reef fishes in Providencia island
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下载链接：
https://datadryad.org/dataset/doi:10.5061/dryad.mcvdncjz9
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资源简介：
Environmental DNA (eDNA) provides a revolutionary method to monitor
species in marine ecosystems from animal DNA present in the water.
Examining the capacity of eDNA to provide accurate biodiversity measures
in species-rich ecosystems such as coral reefs is a prerequisite for their
long-term monitoring. Here, we surveyed a Colombian tropical marine reefs,
Providencia Island using eDNA method. We collected a large quantity of
surface water (30 L per filter) above the reefs and applied a
metabarcoding protocol using three different primer sets targeting the 12S
mitochondrial DNA, specific to vertebrates, Actinopterygii and
Elasmobranchii. The assignment of eDNA sequences to species using a public
reference database allowed detecting the presence of 107 fish species, 106
genera and 73 families in Providencia.
提供机构：
Dryad
创建时间：
2021-06-09
