Rickettsia induces strong cytoplasmic incompatibility in a predatory insect

Rickettsia, a group of intracellular bacteria found in eukaryotes, exhibits diverse lifestyles, with some acting as vertebrate pathogens transmitted by arthropod vectors and others serving as maternally transmitted arthropod endosymbionts, some of which manipulate host reproduction for their own benefit. Two phenotypes, namely male killing and parthenogenesis induction are known as Rickettsia-induced host reproductive manipulations, but it remains unknown whether Rickettsia can induce other types of host manipulation. In this study, we discovered that Rickettsia induced strong cytoplasmic incompatibility (CI), in which uninfected females produce no offspring when mated with infected males, in the predatory insect Nesidiocoris tenuis (Hemiptera: Miridae). Molecular phylogenetic analysis revealed that the Rickettsia strain was related to Rickettsia bellii, a common insect endosymbiont. Notably, this strain carried plasmid-encoded homologues of the CI-inducing factors (namely cifA-like and..., Crossing experiment
 
Treatment
 
Females and males of the Nesidiocoris tenuis OW1 (Rickettsia infected) and OW1tet (cured) lines were crossed in all possible combinations to compare the number of eggs laid and the hatch rates. Males and females were collected separately from newly emerged adults. A 3–6day old female was allowed to mate with a male for 7 days and then transferred to a container with a leaf of Crassula ovata for oviposition. Leaves were replaced every 2 days, and oviposition continued for 6 days. Ten days after the last day of oviposition, hatched nymphs were counted and leaves were dissected to count unhatched eggs. The food source was renewed every 2 days during the crossing experiments.
 
Statistical analysis
 
The total number of eggs laid was analysed using the Kruskal–Wallis test. Egg hatch rates were analysed using a generalised linear mixed model (GLMM) with binomial error and a logit-link function. Each mating pair was assigned a random effect. Based on the resu..., , # *Rickettsia* induces strong cytoplasmic incompatibility in a predatory insect

[https://doi.org/10.5061/dryad.t1g1jwt9b](https://doi.org/10.5061/dryad.t1g1jwt9b)

This dataset includes the data related to the crossing experiment and phylogenetic analysis. Crossing experiment data contains raw data of eggs laid (**NTeggs.csv**), R script for eggs laid (**NTeggs.R**), raw data of egg hatch rate (**NTHatchRate.csv**), R script for egg hatch rate (**NTHatchRate.R**). Phylogenetic analysis data  contains fasta data of 16SrRNA nucleotide sequence (**16SrRNA.fas**) and *cif* gene nucleotide sequence (**cifA-B.fas**).

## **Description of the data and file structure**

### **Crossing experiment**

##### NTeggs.csv

* *treatment*: Mating pair of crossing experiments

RR: the pair of male of *Rickettsia* infected line (OW1) and female of *Rickettsia* infected line (OW1)

RC: the pair of male of *Rickettsia* infected line (OW1) and female of cured line (OW1tet)

CR: the pair of mal...