Next-generation sequencing analysis of macrophage engulfing control or STC1-OE tumor corpses related transcriptomes

Purpose: To explore the transcriptomes changes in macrophage during long term engulfment of control or STC1-OE tumor corpses. Overall design: Methods: Macrophages were loaded with STC1OE or control B16F-F10 corpses at 1 to 5 ratios. After culture for 20 hours, unbound tumor corpses were washed away. Total RNA was isolated using the Direct-zol RNA Miniprep Plus kit (Zymo Research) and mRNA libraries were prepared using the Illumina TruSeq technology. Libraries were then sequenced using an Illumina NovaSeq sequencer at 150?bp, paired end reads with approximately 30?million reads per sample. Four independent experiments were sequenced for each condition.