Chromatin structure and organization in euchromatin: Sequencing of H3.3-HaloTag-incorporated genomic regions in HCT116 cells

The textbook view holds that transcriptionally inactive heterochromatin forms condensed domains, whereas active euchromatin is open and accessible. Using advanced nanoscopic imaging, we revealed condensed euchromatic domains labeled by H3.3-HaloTag in living human cells, challenging the long-standing notion that euchromatin is largely open. These condensed euchromatic domains may contribute to higher-order gene regulation. In this project, we purified H3.3-HaloTag-labeled euchromatin from HCT116 cells, sequenced it, and mapped the corresponding genomic regions.