File S1 - Genetic Analysis of Benzothiophene Biodesulfurization Pathway of Gordonia terrae Strain C-6

Figure S1, Histogram of clusters of orthologous groups (COG) classification. A, RNA processing and modification; B, Chromatin structure and dynamics; C, Energy production and conversion; D, Cell cycle control, cell division, chromosome partitioning; E, Amino acid transport and metabolism; F, Nucleotide transport and metabolism; G, Carbohydrate transport and metabolism; H, Coenzyme transport and metabolism; I, Lipid transport and metabolism; J, Translation, ribosomal structure and biogenesis; K, Transcription; L, Replication, recombination and repair; M, Cell wall/membrane/envelope biogenesis; N, Cell motility; O, Posttranslational modification, protein turnover, chaperones; P, Inorganic ion transport and metabolism; Q, Secondary metabolites biosynthesis, transport and catabolism; R, General function prediction only; S, Function unknown; T, Signal transduction mechanisms; U, Intracellular trafficking, secretion, and vesicular transport; V, Defense mechanisms; W; Extracellular structures; Y, Nuclear structure; Z, Cytoskeleton. Figure S2, MS spectra of the phenolic compound produced by cell extracts of E. coli Rosetta (DE3) conceived with pET28a-bdsABC during BT biodesulfurization. Metabolites of BT in the cell extracts reaction system was extracted directly with ethyl acetate without adjusting its pH to 2.0, and analyzed by GC-MS according to the previously described method [25]. o-hydroxystyrene was identified by MS spectra corresponding to the peak with retention time of 3.67 in the GC profile. The molecular weight of o-hydroxystyrene decreased by 1 (the hydroxyl of o-hydroxystyrene exists in the form of negative ions) due to the extracting procedure. Table S1, The amino acid homology of BT/DBT biodesulfurization enzymes. Table S2, in File S1 Sulfur resource specificity of Gordonia terrae C-6. Table S3, in File S1 Primers used for RT-qPCR. (DOCX)