Fusarium virguliforme Mont-1 isolate:mont-1 Genome sequencing

We sought to improve the Fusarium virguliforme reference genome using third generation sequencing technologies. To do this, we generated a high-quality PacBio single-molecule derived genome, based on 6.5 Gb of sequencing data. This resulted in a 17x coverage of the F. virguliforme genome. Filtered reads were assembled using the long-read optimized assembler Canu, and resultant contigs were error-corrected using 50x Illumina data by Pilon. In total the assembled F. virguliforme genome encompassed 52 mb with 96 contigs, with a N50 of 1.54 mb.