Changes in DNA methylation pattern following intervention in PTSD patients. Homo sapiens

Objective: The purpose of this study was to observe changes in DNA methylation in service members who experienced PTSD. Methods: We compared DNA Methylation profiles in the blood of those with or without PTSD and also compared those with PTSD at both baseline and follow-up with those service members that only developed PTSD at follow-up (3-months after baseline). Blood was collected and analyzed at baseline and follow up. Results: In participants with PTSD, 10 genes were significantly hyper-methylated and 18 were significantly hypo-methylated. In the second analysis, participants that had PTSD at both time points did not present any differentially methylated gene regions. Interestingly, for individuals’ whose PTSD scores increased from baseline to follow-up, there were 8 hypo-methylated genes detected at the baseline. Overall design: Methyl-CpG-Binding Domain (MBD) seq All the sample names starting with 'c' is from control, meaning "no PTSD" 'i' means 'increased' PTSD, and 'p' means 'persistent' PTSD when we compare at time point 2 when we have a reference from at time point 1 (3-months after baseline).