Single-cell multi-omics profiling of PBMCs from SLE patients pre- and post-dual-target (BCMA/CD19) CAR-T therapy

We employed single - cell RNA sequencing combined with BCR/TCR profiling to study PBMCs from 5 SLE patients pre - treatment and 6 months post dual - target (BCMA/CD19) CAR - T therapy. PBMCs were analyzed to characterize dynamic immune cell subsets, BCR/TCR clonal expansion, and SLE - related pathways. This study provides a single - cell landscape of dual - target CAR - T in SLE, offering insights into efficacy and biomarkers. Following dual-target (BCMA/CD19) CAR-T therapy for SLE, PBMCs from 5 patients were collected pre-treatment and 6 months post-treatment, isolated by density gradient centrifugation, and analyzed using scRNA-seq with BCR/TCR profiling.本研究采用单细胞RNA测序（single-cell RNA sequencing）联合BCR/TCR谱分析，对5名系统性红斑狼疮（systemic lupus erythematosus, SLE）患者在双靶点（BCMA/CD19）嵌合抗原受体T细胞（chimeric antigen receptor T-cell, CAR-T）治疗前及治疗后6个月的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）开展研究。通过对上述外周血单个核细胞的分析，本研究旨在解析动态免疫细胞亚群、BCR/TCR克隆扩增以及与系统性红斑狼疮相关的信号通路特征。本研究构建了系统性红斑狼疮患者接受双靶点CAR-T治疗后的单细胞全景图谱，为阐明治疗疗效与潜在生物标志物提供了全新研究视角。 针对系统性红斑狼疮患者的双靶点（BCMA/CD19）CAR-T治疗，本研究采集了5名患者治疗前及治疗后6个月的外周血单个核细胞，经密度梯度离心法分离后，采用单细胞RNA测序（scRNA-seq）联合BCR/TCR谱分析完成检测。