Osteopontin expression identifies a subset of recruited macrophages distinct from Kupffer cells in the fatty liver. [Single cell RNA Seq]

CITE-seq analysis on total CD45+ cells isolated from the livers of mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12, 24 and 36 weeks. We also performed single cell RNA sequencing analysis of the CD45- cells isolated from the livers of the mice fed the SD or WD for 24 and 36 weeks. Overall design: Single cell RNA Seq = Liver CD45+ and CD45- cells derived from mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12, 24 and 36 weeks. Bulk RNA Seq = Kupffer cells, moKCs and Clec4f- macrophages derived from mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12 and 24 weeks.