Data and code from: Multigenerational exposure to high-fat and high-sugar diets exacerbates reproductive distress in an insect model

In our study, we used experimental evolution to determine how high-fat and high-sugar diets affect the fitness of the common house fly Musca domestica, and if multigenerational exposure leads to compensatory metabolic changes. Housefly larvae were reared on a high-fat, high-sugar, or control diet for 51 consecutive generations, whereupon we measured several larval, pupal, and adult life-history traits. To test the direct effects of the diets on life-history traits, we switched the 50th generation of control diet flies onto the experimental diets and measured the same life history traits. We also directly compared the single-generational exposed and the multigenerational evolved lines to determine if the observed diet-induced adverse metabolic effects expanded across generations.