Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9 based approach reveals high transcriptional variation

Inderbitzin, Loosli and colleagues employ a novel method to construct and extensively characterize DNA barcode libraries and apply CRISPR/Cas9 technology for targeted insertion into the safe harbor gene AAVS1 in Jurkat cells. This technique revealed high fluctuations in gene expression in AAVS1, spanning over two logs.