Expression data of hippocampal CA3 from Adora1 knockout and wild type mice

Purpose: The goals of this study are to analyze the mRNAs expression in hippocampal CA3 after Adora1 knockout by High-throughput sequencing. Methods: The brain was dissected and followed by 20 um cryosection onto 20 glass slides. The hippocampus CA3 was isolated from brain slides by using laser capture microdissection. Then the samples were stored on dry ice until RNA extraction by Trizol. The RNAseq was conducted by using Illumina HiSeq2000 platform. Results: By performing RNA sequencing on RNAs isolated from the hippocampus CA3 neurons of AKO and C57 mice at 3-month-old. We found that 51 mRNAs were upregulated, whereas 77 mRNAs were downregulated in AKO mice. Conclusion:We deciphered the underlying neuroprotective mechanisms of A1R knockdown in AD mouse models. By employing the RNA-seq technique, we compared the alterations in gene expression in the hippocampus between AKO and wild-type mice. Adora1 knockout mice (n=3; AKO mice) were compared to wild type mice (n=3). All mice are C57BL/6 background.研究目的：本研究旨在通过高通量测序（High-throughput sequencing）技术，分析腺苷A1受体基因（Adora1）敲除后小鼠海马CA3区的信使核糖核酸（mRNA）表达情况。 实验方法：解剖小鼠脑组织后，制备20 μm冰冻切片并粘贴至20张载玻片上；采用激光捕获显微切割（laser capture microdissection）技术从脑组织切片中分离海马CA3区组织。将分离得到的样本保存于干冰中，直至使用TRIzol试剂完成RNA提取。随后依托Illumina HiSeq2000平台开展RNA测序（RNAseq）实验。 实验结果：对3月龄腺苷A1受体基因敲除（Adora1 knockout, AKO）小鼠与野生型C57BL/6小鼠的海马CA3区神经元RNA进行测序分析，结果显示AKO小鼠体内共有51条mRNA表达上调，77条mRNA表达下调。 研究结论：本研究阐明了腺苷A1受体敲除在阿尔茨海默病（AD）模型小鼠中的潜在神经保护机制。本研究通过RNA测序技术，对比了AKO小鼠与野生型小鼠海马组织的基因表达差异。本次实验中，Adora1敲除小鼠（n=3）与野生型小鼠（n=3）均为C57BL/6背景。