EZH2 Inhibitors Promote ß-like Cell Regeneration in T1D Donor tissue

ß-cells are a type of endocrine cell found in pancreatic islets that synthesize, store and release insulin. Destruction of these cells in type 1 diabetes leads to a lifelong dependence on exogenous insulin administration for survival. Here we employ RNA-seq to examine the promotion of ß-like cell regeneration with EZH2 inhibition in pancreatic ductal epithelial cells and exocrine cells isolated from type 1 diabetic donor tissue. Overall design: Cells treated with EZH2 inhibitors ( Tazemetostat or GSK ) or vehicle control for a total of 48 hours prior to RNA extraction and preparation of RNA sequencing libraries for transcriptomic analysis.