Histone methylation profiles of H3K4me3, H3K9me3, H3K36me3, H4K20me3, and RIOX2 in WT and mdig/mina53 KO MDA-MB 231 breast cancer cells

The mineral dust-induced gene (mdig, also called as MINA or RIOX2) is overexpressed in breast cancer and its high expression correlates with poor overall survival of the patients. Our studies suggested its role in cell motility, invasion and in the demethylation of DNA and histone proteins. Here we studied the global histone methylation profiles of human triple negative breast cancer cells MDA-MB-231 that were knocked out for mdig via CRISPR-Cas 9 gene editing. Altered histone methylation profiles impacted the transcriptional status of key genes implicated in cell growth, motility, invasion and cancer. Examination of 5 different histone modifications in WT and mdig KO MDA-MB 231 cells.