Differential regulation of p62-ubiquitin conjugates in neurons versus astrocytes during cellular stress

This dataset includes quantification of levels of p62, ubiquitin, LC3, p62-ubiquitin colocalization, and/or p62-GFP(LC3) colocalization in neurons or astrocytes under the following paradigms: (1) Lysosomal damage induced with LLOMe treatment (Fig. 1E-G’, S2A in associated manuscript), (2) Pharmacological reduction of ubiquitination with TAK-243 prior to LLOMe treatment (Fig. 2C-D’), (3) 30-minute starvation in Earle’s Balanced Salt Solution (EBSS; Fig. 3B-D’; S2B-C), (4) 4-hour starvation in EBSS (Fig. S2D-E, S3), (5) Pharmacological reduction of ubiquitination with TAK-243 prior to 30 minutes of EBSS starvation (Fig. 4C-D’, S2F-G, S4). Data are provided as .csv files for all figures for broad accessibility. Files are organized by figure number. All experimental data were generated using cocultures of primary mouse astrocytes and neurons; procedures were performed under IACUC-approved protocols. No restrictions apply to data reuse. These data enable independent verification of statistic..., , # Data from: Differential regulation of p62-ubiquitin conjugates in neurons versus astrocytes during cellular stress

Dataset DOI: [10.5061/dryad.z612jm6s2](https://doi.org/10.5061/dryad.z612jm6s2)

## DATASET FILES: Sidibe_et_al.zip

## DESCRIPTION OF THE DATA AND FILE STRUCTURE

### GENERAL INFORMATION

* This dataset contains the numerical values underlying all main figures (Figs. 1-4) and supplementary figures (Figs. S2-S4) in Sidibe et al., \"Differential regulation of p62-ubiquitin conjugates in neurons versus astrocytes during cellular stress\", *PLoS ONE*.
* **File naming convention:**
* Files are organized into folders that are titled with the relevant Figure number
* Files are named by figure panel (e.g., Figure 1E_Neurons_p62_biological replicates.csv). The prefix indicates the figure number and panel, followed by the cell type that is measured (e.g., neurons or astrocytes), followed by the marker that is measured (e.g., p62, ubiquitin, p62-Ub Colocalization, GFP(LC3), p62-..., ,