Supplementary information for: 3D-printable cell crowding device enables imaging of live cells in compression

Figure
S1.
Cell density along the axis of compression increased approximately 20%, from
14.0± 0.5 to 16.6 ± 0.9. Error bars indicate standard error of the mean.
Cells were counted using the maximum intensity projections of the Hoechst
channel before and after crowding

Figure
S2.
Exploded view of the assembled device, with annotation numbers as described
in Tables S1 and S2. Since some regions of this device experience high
localized stress during tension, the 3-D printed components included
structural support features such as side ribs (1)for the base plate and
gussets for the indenter (5, in inset).

Table
S1: 3-D Printed Parts

Table
S2: McMaster-Carr Parts