Human Colorectal Tissue OCT Dataset: Neoplastic and Non-Neoplastic Samples from Chorioallantoic Membrane (CAM) Assays

A commercial OCT system (Telesto II-1325 LR spectral domain OCT (SD-OCT)) was used to imaging two colon cell lines implanted in a chick embryo chorioallantoic membrane (CAM) assay. This is a high-performance imaging system designed for in vivo and ex vivo imaging of biological tissues.

The CAM is a highly vascularized extra-embryonic membrane connected to the developing embryo through an easily accessible circulatory system that allows the successful engraftment of a variety of foreign tissues, such as tumor explants or cancer cell lines. Neoplastic and non-neoplastic tumors were developed from RKO and NCM460 cell lines, respectively.

OCT B-scan images were collected from 30 CAM models, 15 with RKO-cells and 15 with NCM460-cells. The collected images are avalilable in format OCT format (Neoplastic_OCT.zip/Non-neoplastic_OCT.zip), TIFF format (Neoplastic_tiff.zip/Non-neoplastic_tiff.zip), and MAT format (Neoplastic_mat.zip/Non-neoplastic_mat.zip).

Since, the attenuation of near-infrared light in biological samples has proven to be a powerful tool for tissue characterization the attenuation coefficient of light was calculated for each image. This data is available in the Neoplastic_processed.zip and Non-neoplastic_processed.zip folders.