Inhibition of PARP activity affects transcription and promoter histone acetylation level of p300 and PCAF.

(A) Real time qRT-PCR analysis of mRNA accumulation for two acetyltransferases, p300 and Pcaf. Histograms indicate mRNA level of cells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with Actβ or Hprt. (B) Real time qRT-PCR analysis of histone H3 and H4 acetylation of p300 and Pcaf promoter regions. Histograms indicate acetylation level of cells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with an internal region of Actβ. Primers used were described in Materials and Methods. Error bars indicate the standard deviation of data obtained from three independent experiments. NT: control cells, no treatment. *p ≤ 0.05; **p ≤ 0.01.