Effect of knockout of and pathogenic mutations in Hnrnph2 on gene expression in mouse cortex

To investigate the effect of knockout of and pathogenic mutatations in Hnrnph2, we generated mouse lines in which endogenous Hnrnph2 has been knocked out, or 2 pathogenic mutations (R206W, P209L) have been knocked into the endogenous Hnrnph2 using CRISPR Cas-9. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of bulk cortex from male mice aged 8 weeks (knockout and R206W knockin lines) or 3 weeks (P209L knockin line) and age matched littermate controls (n=3 per grpup).