Gut symbiotic microbiota of Paradieuches dissimilis

Deep sequencing of 16s rRNA gene was performed on 69 symbiotic organs of the collected insects to reveal the diversity of microorganisms in the symbiotic organs. DNA was extracted from the symbiotic organ and the V4 variable region of 16S rRNA was amplified by using 515F and 806R universal primers for deep sequencing of Illumina. DNA libraries containing all tagged amplicons and internal controls (bacteriophage phiX) were prepared for paired-end sequencing using the iSeq-100 reagent kit (version 2; Illumina) and sequenced using the Illumina iSeq-100 instrument according to the manufacturer's instructions.