Aedes aegypti Histone Modification Cut&Run

Aedes aegypti adult midguts were interrogated to define histone modifications that occur following exposure to Rift Valley fever virus (RVFV MP12, Phlebovirus riftense, family Phleboviridae) . Midguts were collected at 1, 3 and 7 days post-exposure (dpe) for chromatin immunoprecipitation sequencing via Cleavage Under Targets and Release Using Nuclease (CUT&RUN), using antibodies against H3K27ac and H3K9me3. Our goal was to interrogate regions of active chromatin and enhancers associated with areas of active or repressed gene expression. Sample types are SF (sugar-fed), BF (bloodfed), BFa (bloodfed with 1:1 dilution in conditioned cell culture media plus 1mM ATP) of RVFV (virus in cell culture media diluted 1:1 in blood plus 1mM ATP).