BIOGRID CURATED DATA FOR PUBLICATION: Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

Protein-Protein, Genetic, and Chemical Interactions for Wigge PA (1998):Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. curated by BioGRID (https://thebiogrid.org); ABSTRACT: A highly enriched spindle pole preparation was prepared from budding yeast and fractionated by SDS gel electrophoresis. Forty-five of the gel bands that appeared enriched in this fraction were analyzed by high-mass accuracy matrix-assisted laser desorption/ ionization (MALDI) peptide mass mapping combined with sequence database searching. This identified twelve of the known spindle pole components and an additional eleven gene products that had not previously been localized to the spindle pole. Immunoelectron microscopy localized eight of these components to different parts of the spindle. One of the gene products, Ndc80p, shows homology to human HEC protein (Chen, Y., D.J. Riley, P-L. Chen, and W-H. Lee. 1997. Mol. Cell Biol. 17:6049-6056) and temperature-sensitive mutants show defects in chromosome segregation. This is the first report of the identification of the components of a large cellular organelle by MALDI peptide mapping alone.

蛋白质-蛋白质、遗传以及化学相互作用数据集，针对 Wigge PA（1998）的研究：通过基质辅助激光解吸/电离（MALDI）质谱分析酵母纺锤体极。该数据集由 BioGRID（https://thebiogrid.org）整理；摘要：从出芽酵母中制备了一种高度富集的纺锤体极，并通过 SDS 凝胶电泳进行分离。在分离的这部分中，分析了 45 条富集的凝胶带，通过高精度质量矩阵辅助激光解吸/电离（MALDI）肽段质量映射与序列数据库搜索相结合的方法。这识别出了十二种已知的纺锤体极成分以及另外十一种未曾定位到纺锤体极的基因产物。免疫电子显微镜将其中八种成分定位到纺锤体的不同部分。其中一个基因产物 Ndc80p 与人类 HEC 蛋白（Chen, Y., D.J. Riley, P-L. Chen, and W-H. Lee. 1997. Mol. Cell Biol. 17:6049-6056）具有同源性，且温度敏感突变体在染色体分离方面表现出缺陷。这是首次通过 MALDI 肽段映射独立识别大型细胞器成分的报道。