Seawater carbonate chemistry and environmental DNA and RNA Shedding and Persistence

In the present study, we conducted mesocosm experiments to determine the shedding and decay rate constants of eDNA and eRNA in M. gigas (Magallana [Crassostrea] gigas) using mitochondrially encoded tRNA leucine 1 (mt-tl1) marker at various partial pressures of CO2 in seawater. To our knowledge, this is the first study manipulating seawater pH using CO2. We developed a sensitive and specific quantitative PCR-based assay to detect M. gigas eDNA and eRNA.