Transcription profiling of lettuce in response to UV-B radiation. Transcription profiling of lettuce in response to UV-B radiation

UV-B radiation is one of the major environmental stresses that triggers a variety of plant responses. However, limited information is available regarding plant biological reactions which help to circumvent the potentially harmful effects of UV-B radiation in lettuce (Lactuca sativa L.). In this study, RNA-seq was performed to identify differentially expressed genes in response to UV-B radiation. Overall design: The lettuce cultivar Shenxuan 5 was used in this study. Four week-old healthy seedlings were exposed to UV-B radiation (310 nm light-emitting diode, T9E31C, Seoul Optodevice, 0.3Wm-2). Devices for supplemental UV-B radiation were suspended above and perpendicular to the planted rows (rows oriented in an east-west direction to minimize shading). Samples were collected at four time points: 0h, 6h, and 24h of UV-B radiation and 12h of recovery under normal conditions after 24h of UV-B radiation. Two biological replicates were used for each time points