Single-cell transcriptome profiles of wild-type and IRF8KO microglia [scRNA-seq]

The role of the lineage-determining transcription factor IRF8 in microglia remains unelucidated. We analyzed the transcriptome diversity of wild-type and IRF8KO microglia. Deep-sequencing and subsequent differential analysis revealed the significance of the IRF8-dependent transcription on microglial heterogeneity and self-renewing. This study provides a novel insight into understanding IRF8-mediated transcriptional programs in microglia. Transcriptome profiles of adult WT and IRF8KO microglia were analyzed at a single-cell level using 10xGenomics.小胶质细胞中谱系决定转录因子IRF8（Interferon Regulatory Factor 8）的功能仍有待阐明。本研究针对野生型（wild-type, WT）与IRF8敲除（IRF8 knockout, IRF8KO）小胶质细胞的转录组多样性展开分析。通过深度测序及后续差异分析，本研究揭示了IRF8依赖的转录过程对小胶质细胞异质性与自我更新的关键作用。本研究为解析小胶质细胞内IRF8介导的转录程序提供了全新视角。本研究采用10xGenomics平台，对成年野生型与IRF8KO小胶质细胞的转录组图谱开展了单细胞水平的分析。