Summary of the on-off protocol of the NF-κB system in WT and A20 KO cells used for in silico experiments.

A simple protocol with a continuous TNF stimulation (0–120min) followed by a TNF washout (120–720min) for which measured variables are: IKKa, nuclear NF-κB, A20, total IκBα protein denoted by IκBα* (IκBα* = IκBα + 1 − NFκB), and IκBα mRNA. For each variable the measurement times used for the sensitivity-based identifiability analysis and reduced model fitting are given.