Long-Term Hepatitis B Virus Infection Induces Cytopathic Effects in Primary Human Hepatocytes, and Can be Partially Reversed by Antiviral Therapy

Transcriptomic analysis was then applied to identify genes or pathways that could be rescued by RNAi. Overall design: A combination of two siRNA (siHBV, 100 nM) to degrade all kinds of HBV RNAs (targeting both HBs and HBx sequences) was transfected by commercial reagents at 7 days post HBV infection, and the cells were collected for RNA-seq 6 days later.