PHD inhibition upregulates glycolytic pathway in OGD

To elucidate the mechanism of enarodustat pretreatment-induced protection against oxygen-glucose deprivation (OGD), we have we have employed whole genome microarray expression profiling as a discovery platform to identify genes upregulated by enarodustat exposure. HK2 cells were pretreated with either vehicle (DMSO) or enarodustat for 24 hours prior to OGD for 16 hours. Pathway analysis revealed upregulated glysolysis pathway. A microarray analysis of 3 groups (sham: 24 h DMSO pretreatment + 16 h in normal medium, ctrl: 24 h DMSO pretreatment + 16 h OGD, enarodustat: 24 h enarodustat pretreatment+ 16 h OGD, n=3 for each group) of HK2 cells was conducted.