Super black plumage is prevalent among Lepidothrix manakins

Super black plumage has been observed across the avian phylogeny, yet few studies have examined the prevalence of super black plumage at the species and subspecies levels. One group where additional super black taxa likely exist is manakins in the genus Lepidothrix. Previous work on these sexually dichromatic taxa showed that male Lepidothrix velutina (Velvety Manakins) have super black plumage, and we observed that males of several other Lepidothrix taxa have black plumage with a velvety appearance that is often associated with super black barbule morphologies. Here, we combine spectrophotometry with scanning electron microscopy to examine the occurrence of super black plumage throughout Lepidothrix. We determine how the barbules of super black taxa differ structurally from barbules of black taxa that lack super black plumage. Our results show that five species and seven subspecies of Lepidothrix have super black plumage, that barbules of back feathers are wider and closer together in super black taxa than in black taxa, and that the super black plumage trait appears to have evolved multiple times independently in the genus. We also show that olive green immature males of super black taxa exhibit wider barbules that are closer together than their counterparts, suggesting the development of super black plumage begins before males molt into their definitive adult plumage.