Isolated VNCs show patterned GCaMP activity.

A) Expression pattern of GCaMP in the OK371-Gal4 (vGlut) driver line. Regions where motoneuron dendrites overlap were chosen as regions of interest (ROI) to monitor segmental calcium levels over time. A ROI was also drawn outside of the VNC for background normalization (upper left corner). The Drosophila VNC is symmetrical across the midline (dotted white line). L/R HS = left and right hand side, respectively. B, C) Activity patterns observed from the left and right hand side of the same VNC. Waves starting at the posterior end of the VNC (FW, forward locomotion) and waves starting at the anterior end of the VNC (BW, backward locomotion) are evident in both sides whilst unilateral activity (turning, only visible in panel B) is only seen one side (labelled LHS).