AFLPseq Nanopore FASTQ read data Adonis volgensis DC. and Adonis fucensis (Ranunculaceae)

The here presented multi-locus Nanopore read data comprises 11 Adonis volgensis DC. samples, as well as one newly described A. fucensis sample (Ranunculaceae). The samples were prepared for sequencing using the AFLPseq genome-reduction method as proposed by Dorfner et al. (2022, DOI: https://doi.org/10.1002/aps3.11484), with the addition of conducting a double digest approach using the restriction enzymes MseI and EcoRI and the 'Ligation by Sequencing LSK109' protocol by Oxford Nanopore Technologies. With the SLANG pipeline (Dorfner et al. 2022), 486 orthologous loci were inferred, containing 2,944 SNPs. After calculation of pair-wise Nei distances, a PCoA plot demonstates the clear separation between the two taxa; with A. fucensis on the right sight of the PCo axis 1, which accounts for 20.8% of the total variation in the data set. Additionally, PCo axis 2 (15.0% of the total variation) shows a clear geographical separation within A. volgensis, with accessions of this species from Romania (sometimes consideres as being an independent species, A. transsilvanica) on the positive and accessions from Russia and Kazakhstan on the negative side of the axis. In addition to morphological differences, we propose that A. fucensis should be treated as an independent species.