Lyons & McClelland 2022

Naming Legend for Lyons & McClelland 2022 Data files.

ha = highlander

la= lowlander

wn = warm normoxia

ch = cold hypoxia

r = resting

ps = vo2max

All analyses were performed using lme4 package in R v.4.0.0. 

We use 2-way analysis of variance to assess main effects of population (highlander vs lowlander) and either acclimation environment (warm normoxia vs cold hypoxia), or activity condition (resting vs vo2max).

We also used a 3-way analysis of variance to assess population, acclimation and activity condition where appropriate.

Data was collected over the course of 2020-2021 at McMaster University. 

Below are breakdown of the figures and tables and the data files accociated with each. 

Figure 1. Rate of glycerol release from inguinal white adipose tissue (ingWAT).

:Lyons & McClelland 2022 white adipose tissue glycerol release.csv

Figure 2. Plasma concentrations of glycerol (A), non-esterified fatty acids (NEFA) (B), and triglycerides (TG) at rest and at cold-induced V̇O2max (C).

:Lyons & McClelland 2022 rest vs vo2max plasma glycerol.csv

:Lyons & McClelland 2022 rest vs vo2max plasma fatty acids.csv

:Lyons & McClelland 2022 rest vs vo2max plasma triglycerides.csv

Figure 3. Plasma flow rate (A) non-esterified fatty acid (NEFA) delivery rate (B) and triglyceride (TG) delivery rate (C) at cold-induced V̇O2max in hypoxia.

:Lyons & McClelland 2022 vo2max plasma delivery rate.csv

Figure 4. Intramuscular triglyceride (TG) concentration during rest and immediately after cold-induced V̇O2max. 

:Lyons & McClelland 2022 rest vs vo2max imtg.csv

Figure 5. Relative protein abundance of fatty acid translocase (FAT/CD36) in brown adipose tissue. 

:Lyons & McClelland 2022 brown adipose tissue cd36.csv

Figure 6. Total protein abundance of fatty acid translocase (FAT/CD36) (A), sarcolemmal fraction FAT/CD36 abundance at cold-induced V̇O2max (B) and fatty acid binding protein (FABP) abundance (C) in the gastrocnemius.

:Lyons & McClelland 2022 whole gastrocnemius cd36.csv

:Lyons & McClelland 2022 gastrocnemius sarcolemmal cd36.csv

:Lyons & McClelland 2022 gastrocnemius fatty acid binding protein.csv

Figure 7. Apparent maximal enzyme activities (Vmax) of carnitine palmitoyl transferase (CPT) in isolated muscle mitochondria (A), and ß-hydroxyacyl-CoA dehydrogenase (HOAD) (B), citrate synthase (CS) (C), and cytochrome c oxidase (COX) (D) in the gastrocnemius. 

:Lyons & McClelland 2022 gastrocnemius cpt enzyme activity.csv

:Lyons & McClelland 2022 gastrocnemius hoad and cox enzyme activity.csv

:Lyons & McClelland 2022 gastrocnemius citrate synthase enzyme activity.csv

Figure S1. Exogenous fatty acid oxidation rates of soleus muscle.

:Lyons & McClelland 2022 soleus exogenous lipid oxidation.csv

Table 1. Body mass, inguinal white adipose tissue (ingWAT) mass and ingWAT mass relative to body mass of first-generation laboratory born and raised highland and lowland deer mice (Peromyscus maniculatus), acclimated to control warm normoxia (23oC, 12 kPa O2) or cold hypoxia (5oC, 12 kPa O2) conditions.

:Lyons & McClelland 2022 relative white adipose tissue mass.csv

Table 2. Concentrations of individual plasma fatty acids (µmol L-1) of first-generation laboratory born and raised highland and lowland deer mice (Peromyscus maniculatus), acclimated to control warm normoxia (23oC, 12 kPa O2) or cold hypoxia (5oC, 12 kPa O2) conditions, during rest and cold-induced V̇O2max.

:Lyons & McClelland 2022 rest vs vo2max plasma fatty acids.csv